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We Beforehand identified PS1 mutants Lively from the absence of NCT [29]. These mutants possessed two missense mutations in PS1: S438P on the ninth transmembrane domain together with a single mutation distributed throughout the molecule. These PS1 mutations Increased and modulated the γ-secretase action and lowered the ratio of prolonged Aβs [32]. We then isolated Aph1 or Pen2 mutants in a position to mature inside the absence of NCT. immediately after we had screened cells with Pen2 or Aph1 mutations and the PS1 S438P mutation, we discovered 13 Aph1 mutants that grew during the absence of NCT (Table one and Figure 1).
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